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Using molecular modeling methods we determined possible mutual positions of two dCas9-proteins at the target locus of genomic DNA, which allow optimal interaction of the domains of the split-enzyme attached to them.

Abstract For the diagnosis of many humans, animals and plant diseases, environmental monitoring, highly sensitive, specific, fast and easy-to-use diagnostic methods for the detection of nucleic acids of pathogens are required.

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In this work, we have designed and studied the applicability of a biosensor system based on a combination of the ability of dCas9-proteins to bind to target DNA.

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Notifications View Subscribe. Soranno A. Makarova K. MacKay M. Supporting agencies: The research was funded by Russian Foundation for Basic Research, project number For the diagnosis of many humans, animals and plant diseases, environmental monitoring, highly sensitive, specific, Prostitutes Leninskiye Gory and easy-to-use diagnostic methods for the detection of nucleic acids of pathogens are required. Porter J.
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